This site is intended for healthcare professionals
Journals
  • Home
  • /
  • Journals
  • /
  • Cystic fibrosis
  • /
  • Identification of the cystic fibrosis gene: clonin...
Journal

Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA.

Read time: 1 mins
Published:8th Sep 1989
Author: <p>Riordan JR, Rommens JM, Kerem B, Alon N, Rozmahel R, Grzelczak Z, et al.</p>
Source: Science
Availability: Pay for access, or by subscription
Ref.:Science. 1989 Sep 8;245(4922):1066-73.
DOI:10.1126/science.2475911

Overlapping complementary DNA clones were isolated from epithelial cell libraries with a genomic DNA segment containing a portion of the putative cystic fibrosis (CF) locus, which is on chromosome 7. Transcripts, approximately 6500 nucleotides in size, were detectable in the tissues affected in patients with CF. The predicted protein consists of two similar motifs, each with (i) a domain having properties consistent with membrane association and (ii) a domain believed to be involved in ATP (adenosine triphosphate) binding. A deletion of three base pairs that results in the omission of a phenylalanine residue at the center of the first predicted nucleotide-binding domain was detected in CF patients.

Read abstract on library site

Access full article