Chronic Myeloid Leukemia Knowledge Centre

CML Management

Ongoing Management

The goals of therapy in patients with chronic myeloid leukaemia (CML) are to:

The management of CML is based on meeting certain therapeutic milestones within a specified period of time (Table 1).

Table 1. Haematological, Cytogenetic, and Molecular Monitoring
 DefinitionMonitoring
Haematological response (HR)
Complete HR (CHR):
  • Platelet <450 × 109/L
  • WBC count <10 × 109/L
  • Differential without immature granulocytes and with <5% basophils
  • Nonpalpable spleen
  • Every 2 weeks until CHR has been achieved and confirmed*
  • Followed by monitoring every 3 months unless otherwise required
Cytogenetic response (CyR)
  • Complete: Ph+ 0
  • Partial: Ph+ 1%-35%
  • Minor: Ph+ 36%-65%
  • Minimal: Ph+ 66%-95%
  • None: Ph+ >95%
  • At least every 6 months until complete response has been achieved and confirmed↑
  • Followed by monitoring at least every 12 months
Molecular response (MR)
  • BCR-ABL transcript level expressed as a percentage of a control gene according to the standardised international scale?
    - Complete: nonquantifiable and nondetectable transcript level
    - Major: ≤0.10% transcript level
  • Every 3 months once CCyR is achieved§
  • Mutational analysis for failure, suboptimal response, or substantial transcript level increase

*Complete HR should be confirmed on 2 subsequent occasions.
↑Complete CyR should be confirmed on 2 subsequent occasions.
?The international scale as proposed by Hughes et al.1
§ Major molecular response should be confirmed on 2 subsequent occasions.
WBC, white blood cell.
Adapted with permission from Baccarani et al.2

Haematological Response

A haematological response (HR) includes reducing platelet counts to < 450 × 109/L and WBC counts to < 10 × 109/L, decreasing the basophil level to < 5%, eliminating immature granulocytes from the peripheral blood, and eradicating the signs and symptoms of disease (Table 1).1-3 It is recommended that HR be evaluated every 2 weeks until a complete HR has been achieved and confirmed in 2 subsequent occasions, followed by monitoring every 3 months.2

Cytogenetic Response

Cytogenetic response (CyR) is an important goal of therapy, with progression-free survival benefits for patients. CyR is based on analysis of bone-marrow aspirates and the reduction or elimination of Philadelphia chromosome-positive (Ph+) cells. A complete cytogenetic response (CCyR) is defined by the absence of Ph+ cells from bone marrow; a partial CyR by the presence of 1%-35% Ph+ cells; a minor CyR by the presence of 36%-65% Ph+ cells; a minimal CyR by the presence of 66%-95% Ph+ cells; and no CyR by the presence of >95% Ph+ cells in bone marrow (Table 1).1,2 A cytogenetic examination is recommended before treatment, and at least every 6 months until a CCyR has been achieved and confirmed in 2 subsequent occasions, followed by monitoring every 12 months during treatment.2

Molecular analytic techniques, such as fluorescence in situ hybridisation (FISH) or reverse transcriptase polymerase chain reaction (RT-PCR), also may be used to detect the presence or absence of BCR-ABL and monitor disease progression.4 Although the prognostic role of these techniques continues to be clarified, there is substantial evidence that major reductions in the level of BCR-ABL transcripts correlate with progression-free survival in patients with CML who achieve a CCyR.5 For patients receiving therapy for CML, the magnitude of reduction in tumour burden is a key prognostic indicator. Moreover, most patients treated with Glivec® (imatinib) achieve a CCyR, thus increasing the importance of molecular monitoring of residual disease to further stratify response to treatment and to promptly detect risk of loss of response.6,7  Major molecular response (MMR) has been defined as a ≥3-log decrease in BCR-ABL:BCR mRNA transcripts as measured by quantitative RT-PCR, or a BCR-ABL:ABL ratio <0.10% (Table 1).1,2 Undetectable or unquantifiable levels of BCR-ABL constitute a complete molecular response (CMR)2. The definition of response, however, is evolving based on differences in prognostic value. Molecular response should be evaluated every 3 months once CCyR has been achieved, and an MMR should be confirmed on 2 subsequent occasions. Mutational analysis is highly recommended in case of failure, suboptimal response, or BCR-ABL transcript level increase on at least 2 subsequent occasions.2

Response Definition and Monitoring
For the minority of patents with CML who do not respond to Glivec therapy, lack of response to treatment at different time points can be defined as failure, suboptimal, or warnings (Table 2).2

Table 2. Response to Glivec in Patients With Chronic-Phase CML
Failure
  • Glivec treatment at the current dose is no longer appropriate
  • Dose increase or other treatments are recommended
Suboptimal Response
  • Long-term outcome at current dose not likely to be favourable
  • Dose escalations or other treatments are recommended
Warnings
  • Standard dose Glivec may not be the best option
  • "Warnings" are flexible and do not necessarily mean an action needs to be taken
  • More careful monitoring is required
  • Dose escalation or other treatments are recommended

Adapted with permission from Baccarani et al. 2
A response is defined as failure when treatment at the current dose is no longer appropriate for the patient and dose escalation or other treatments are required. Suboptimal response indicates that the patient may still have substantial benefit from treatment continuation; however, long-term outcome of the treatment would not likely be as favourable. Dose escalation or other treatment is recommended for patients with a suboptimal response. Warning is indicated when the standard dose of the treatment may not be the best option. Although warnings are flexible and do not necessarily mean that action needs to be taken, a more careful monitoring and dose escalation or other treatments are highly recommended. The criteria proposed by the European LeukemiaNet for failure, suboptimal response, and warning are listed in Table 3.2

Table 3. Criteria for Failure, Suboptimal Response, and Warnings at Different Time Points During Glivec Treatment
TimeFailureSuboptimal ResponseWarnings
Diagnosis    
  • High risk
  • Del9q+
  • Additional chromosomal abnormalities in Ph+ cells
3 months No HR Any HR < CHR  
6 months No CHR or CyR Any CyR < PCyR  
12 months < PCyR Any CyR > PCyR and < CCyR Any MR < MMR
18 months Any CyR < CCyR Any MR < MMR  
Any time
  • Loss of CHR
  • Loss of CCyR
  • Mutation with high insensitivity to Glivec
  • Additional chromosomal abnormalities in Ph+ cells
  • Loss of MMR
  • Mutation with low insensitivity to Glivec
  • Increase in BCR-ABL level
  • Other chromosomal abnormalities in Ph-cells

HR, haematological response; CHR, complete haematological response; PCyR, partial cytogenetic response; CCyR, complete cytogenetic response; MR, molecular response; MMR, major molecular response.
Reprinted with permission from Baccarani et al.2

References:
1. Hughes T, Deininger M, Hochhaus A, et al. Monitoring CML patients responding to treatment with tyrosine kinase inhibitors: review and recommendations for harmonizing current methodology for detecting BCR-ABL transcripts and kinase domain mutations and for expressing results. Blood. 2006;108:28-37.
2. Baccarani M, Saglio G, Goldman J, et al. Evolving concepts in the management of chronic myeloid leukemia. Recommendations from an expert panel on behalf of the European LeukemiaNet. Blood. 2006;108:1809-1820.
3. Kantarjian H, Sawyers C, Hochhaus A, et al. Hematologic and cytogenetic responses to imatinib mesylate in chronic myelogenous leukemia. N Engl J Med. 2002;346:645-652.
4. National Comprehensive Cancer Network. NCCN Clinical Practice Guidelines in OncologyTM. Available at: http://www.nccn.org/professionals/. Accessed July 19, 2006.
5. Hughes TP, Kaeda J, Branford S, et al. Frequency of major molecular responses to imatinib or interferon alfa plus cytarabine in newly diagnosed chronic myeloid leukemia. N Engl J Med. 2003;349:1423-1432
6. Branford S, Rudzki Z, Harper A, et al. Imatinib produces significantly superior molecular responses compared to interferon alfa plus cytarabine in patients with newly diagnosed chronic myeloid leukemia in chronic phase. Leukemia. 2003;17:2401-2409.
7. Paschka P, Muller MC, Merx K, et al. Molecular monitoring of response to imatinib (Glivec) in CML patients pretreated with interferon alpha. Low levels of residual disease are associated with continuous remission. Leukemia. 2003;17:1687-1694.

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